Lab : Biomimetic and Environmental Materials
Office : 82-54-279-9505 / Lab : +82-54-279-9528
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Postdoctoral Researcher, Environmental and Energy Institute of Technology (EEIT), POSTECH, 2006-2007 
Postdoctoral Researcher, Material Research Laboratory (MRL) & Marine Science Institute (MSI), 
University of California,Santa Barbara (UCSB), 2007-2009 
Otis-William Postdoctoral Fellow in Bioengineering, Material Research Laboratory (MRL) & Marine Science Institute 
(MSI),University of California, Santa Barbara (UCSB), 2009-2011

Research Interests

Research Areas

Biomimetics : “Designing biomedical materials by mimicking marine materials”

Marine microenvironment has much common with human body. Both systems are naturally saline, experience variation of control over fluid flow, fouling by flowing via macromolecules, and degradation of organics via cellular-level activities. These are just a few examples of matching biological and mechanical events both present in the human body and the marine environment. Understanding physics and chemistry of marine biomaterials will provide the scientists useful insights for designing biomaterials for biomedical application. Isolation and characterization of the load bearing precusors in marine biomaterials is a prerequisite for mimicking those materials. Therefore, we aim to isolate, to produce, and to characterize of novel load bearing biomaterials from marine organisms and material properties of these biomaterials will be further investigated by collaborative research with materials and physics departments

Manufacturing biomimetic environmentally friendly materials

Biomimetic approaches will help inspire new generations of building blocks and subsequent production of engineered materials. We seek to produce new intriguing building blocks for material fabrication by biotechnological methods. The building blocks can be assembled and tailored for fabricating materials of multifunctionality in many applications. X-ray diffraction and imaging studies at the synchrotron facility in Pohang accelerator laboratory (PAL) may reveal intriguing insights about the assembly of the engineered materials.

Characterizing the properties and performance of biomimetic material

Here, we seek to understand the properties and performance of biomimetic environmentally friendly materials. Mechanical and biomedical properties can be investigated with various mechanical and biotechnological techniques. The interfaces of the materials can be studied with Electron microscopes (SEM, TEM), X-rays (Synchrotron, XRD), Atomic Force Microscope (AFM) and Surface Force Apparatus (SFA). Especially, intermolecular interaction between the interfaces is one of the most important forces for material assembly. An apparatus most often used for direct measurements of the forces of interaction between two interfaces is the surface force apparatus (SFA). The SFA is an instrument that measures the magnitude and distance of the intermolecular forces between two surfaces by approaching, retracting or shearing from one another.

Selected Publications

1.Hwang DS, Yoo HJ, Jun JH, Moon WK, Cha HJ. expression of functional recombinant mussel adhesive protein Mgfp-5 in Escherichia coli. Appl. Environ. Microbiol. 2004;70:3352-9.
2.Lim HJ, Kim YK, Hwang DS, Cha HJ. expression of functional human transferring in stably transfected Drosophila S2 Cells. Biotechnol. Prog. 2004;20:1192-7.
3.Hwang DS, Gim Y, Cha HJ. expression of functional recombinant mussel adhesive protein type 3A in Escherichia coli. Biotechnol. Prog. 2005;21:965-70.
4.Hwang DS, Gim Y, Kang DG, Kim YK, Cha HJ. Recombinant mussel adhesive protein Mgfp-5 as a cell adhesion biomaterial. J. Biotechnol. 2007;127:727-35.
5. Li L, Kim YS, Hwang DS, Seo JH, Jung HJ, Du J, Cha HJ. High and Compact Formation of Baculoviral Polyhedrin-Induced Inclusion Body by Co-expression of Baculoviral FP25 in E.coli
6.Wei Q, Jung HJ, Hwang DS, Hwang BH, Gim Y, Cha HJ. Escherichia coli-based expression of functional novel DNA-binding histone H1 from Carassius auratus. Enzyme Microb. Tech. 2007; 40:1484-90.
7.Hwang DS, Gim Y, Yoo HJ, Cha HJ. Practical recombinant hybrid mussel bioadhesive fp-151. Biomaterials 2007 28:3560-8.
8.Hwang DS, Sim SB, Cha HJ. Novel cell adhesion biomaterial based on mussel adhesive protein fused with RGD peptide. Biomaterials 2007 28:4039-46.
9.Jung HJ*, Hwang DS*, Wei Q, Cha HJ. Carassius auratus-originated recombinant histone H1 c-terminal peptide as Gene delivery material. Biotechnol. Prog. 2008 24:17-22. *equal contribution
10.Cha HJ, Hwang DS, Lim S. Development of bioadhesives from marine mussels. Biotechnol J 2008 3:631-8
11.Kim DI, Hwang DS, Kang DG, Kim JYH, Cha HJ. Enhancement of Mussel Adhesive Protein Production in Escherichia coli by Co-expression of Bacterial Hemoglobin. Biotechnol Prog 2008 24:663-6
12. Gim Y, Hwang DS, Lim S, Song, YH, and Cha HJ. Production of Fusion Mussel Adhesive fp-353 in Escherichia coli. Biotechnol Prog 2008, 24, 1272-7.
13. Hwang DS, Kim KR, Lim S, Choi YS, Cha HJ. Recombinant mussel adhesive protein as a gene delivery material. Biotechnol. Bioengineer. 2009, 102, 616-23.
14. Cha HJ, Hwang DS, Lim S., White JD, Matos-Perez CR, Wilker JJ. Bulk adhesive strength of recombinant hybrid mussel adhesive protein. Biofouling, 2009, 25, 99-107.
15. Zhao H, Sagart J, Hwang DS, Waite JH . Glycosylated hydroxytryptophan in a mussel adhesive protein from Perna viridis. J Biol Chem 2009, 283,23344-52.
16.Hwang DS, Waite JH, Tirrell M. Promotion of osteoblast proliferation on complex coacervation-based hyaluronic acid – recombinant mussel adhesive protein coatings on titanium, Biomaterials, 2010,31, 1080-4.
17. Hwang DS*, Zeng HB*, Srivastava S, Krogstad DV, Tirrell M, Iraelachvili JN, Waite JH. Viscosity and interfacial properties in a mussel inspired adhesive coacervate, Soft Matter, 2010, 6, 3232-6,*equal contribution
18. Zeng HB*, Hwang DS *, Iraelachvili JN, Waite JH. Strong Reversible Fe3+-mediated Bridging between Dopa-Containing Protein Films in Water Proc. Nat. Acad. Sci. U. S. A., 2010, 107,12850-3, *equal contribution
19. Hwang DS*, Zeng HB*, Masic A, Harrington MJ, Iraelachvili JN, Waite JH. Protein- and metal-dependent interactions of a prominent protein in mussel adhesive plaques, J. Biol. Chem., 2010, 285,25850-8, *equal contribution
20.Choi BH, Choi YS, Hwang DS, Cha HJ.Facile Surface Functionalization with Glycosaminoglycans by Direct Coating with Mussel Adhesive Protein, Tissue Engr., 2012,18,71-9.
21. Lu Q, Hwang DS, Yan L, Zeng H. Molecular interactions of mussel protective coating protein, mcfp-1, from Mytilus californianus. Biomaterials, 2012, 33,1903-11.
22. Hwang DS, Zeng H, Lu Q, Israelachvili JN, Waite JH. Adhesion mechanism in dopa-deficient foot protein from green mussels. Soft Matter, 2012, 8, 5640-5648.
23. Hwang DS, Harrington MJ, Lu Q, Masic A, Zeng H, Waite JH. Mussel foot protein-1 (mcfp-1) interaction with titania surfaces. J. Mater. Chem.,2012, 22, 15530-3.
24. Hwang DS, Waite JH. Three intrinsically unstructured mussel adhesive proteins, mfp-1, mfp-2, and mfp-3: analysis by circular dichroism. Protein Sci., 2012, 21,1689-95.
25. Lu Q, Danner E, Waite JH, Israelachvili JN, Zeng H, Hwang DS. Adhesion of mussel foot proteins to different substrate surfaces. J. Royal Soc. Interface, 2013, in press.
26. Heo J, Kang T, Jang SK, Hwang DS, Sprull JM, Killops KL, Waite JH, Hawker CJ. Improved Performance of Protected Catecholic Polysiloxanes for Bioinspired Wet Adhesion to Surface Oxides. J. Am. Chem. Soc., 2013, in press