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IBB POSTECH

Faculty

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Lab : Molecular Neurophysiology
Office : + 82-54-279-2297 / Lab :+ 82-54-279-5984
Homepage : mnp.postech.ac.kr
E-Mail :

Profile

1989
1982-1985

1989-1991
1997-1998

Ph.D., University of Massachusetts, Amherst
Research Investigator, Genetic Engineering Research Center,
Korea Institute of Science and Technology
Postdoctoral Fellow, Lab. Molecular Neurobiology, Cornell University Medical College
Visiting Scientist, Dept. of Physiol. & Biophys., University of Washington


Research Interests

Molecular Neurophysiology
Research interests in this laboratory aim at the elucidation of the physiological and molecular regulation of neuronal signal system. The major stream of research involves the modulation of second messengers in neuronal cells, regulation of neurotransmitter secretion, and transcriptional regulation of the enzymes involved in the biosynthesis of neurotransmitters. For the purpose, two major approaches are carried out; one is the cellular biological aspect and the other is themolecular biological aspect. In the field of cell biology, the characterization of receptors and ion channels, cross-talk between receptor signalings, the interaction between receptor and ion channels have been investigated. Since extracellular signals on neuronal cells eventually trigger secretion of neurotransmitters, we also study the regulatory mechanism in the exocytotic processes of neurotransmitters. Recently signalings involved in circadian rhythm, neurological disease such as manic depression and schizophrenia have risen as new research interests. With the basic knowledge of receptors, ion channels and their signalings, we are investigating drug development by screening specific antagonists against receptors and ion channels. Through a molecular biology approach, we focus on the transcriptional and translational regulations of neurotransmitter synthesizing enzymes including tyrosine hydroxylase, dopamine beta-hydroxylase, choline acetyltransferase and serotonin N-acetyltransferase. Identification and cloning of regulatory factors involved in those gene expressions are proceeded. We are also searching specific genes expressed in circadian rhythm and neuronal cell differentiation. In addition, cloning of novel receptors and functional analysis is performed.

Selected Publications

1.Suh BC, Kim MJ, Choi G, Choi KY, Han JK, Chung SK and Kim KT (2000) Differential stereoselectivity of D- and L-myo-inositol 1,2,4,5-tetrakisphosphate binding to the inositol 1,4,5-trisphosphate receptor and 3-kinase. Neurochem. Intl. 37, 47-52.

2.Kim YJ, Hur EM, Park TJ and Kim KT (2000) Nongenomic inhibition of catecholamine secretion by 17 beta-estradiol in PC12 cells. J. Neurochem. 74(6), 2490-2496

3.Kim KT, Koh DS, and Hille B (2000) Loading of oxidizable transmitters into secretory vesicles permits carbon-fiber amperometry. J. Neurosci. 20: RC101 (1-5)

4.Oh KS, Park TJ, Choi BH, Lee DK, Lee TK and Kim KT (2000) Inhibition of nicotinic receptor-mediated catecholamine secretion by the water extract from Dryobalanops aromatica in bovine adrenal chromaffin cells. Pharmacol. Res. 42, 559-564

5.Suh BC, Kim TD, Lee IS and Kim KT (2000) Differential regulation of P2Y11 receptor-mediated signaling to phospholipase C and adenylyl cyclase by protein kinase C in HL-60 promyelocytes. Br. J. Pharmacol. 131, 489-497

6.Choi SY, Ha H and Kim KT (2000) Capsaicin inhibits platelet activating factor-induced cytosolic Ca2+ increase and superoxide production. J. Immunol. 165, 3992-3998

7.Suh BC, Lee H, Lee IS and Kim KT (2001) Modulation of H2 histamine receptor-mediated cAMP generation and granulocytic differentiation by extracellular nucleotides via activation of protein kinase C. J. Leukoc. Biol. 69(1), 169-176

8.Park TJ, Suh H, Kang BJ and Kim KT (2001) Noncompetitive inhibition by camphor of nicotinic acetylcholine receptors. Biochem. Pharmacol. 61, 787 – 793

9.Choi SY, Kim YH, Lee YK and Kim KT (2001) Chlorpromazine inhibits store-operated calcium entry and subsequent orepinephrine secretion in PC12 cells. Br. J. Pharmacol. 32, 411-418

10. Hur EM, Park TJ and Kim KT (2001) Coupling of L-type voltage-sensitive calcium channels to P2X2 purinoceptors in PC12 cells. Am. J. Physiol. 280, C1121-C1129

11. Suh BC, Kim JS, Namgung U, Han S and Kim KT (2001) Selective inhibition of b2-adrenergic receptor-mediated cAMP generation by activation of the P2Y2 receptor in mouse pineal gland tumor cells. J. Neurochem. 77, 1475-1485

12. Suh BC, Kim JS, Namgung U, Ha H and Kim KT (2001) P2X7 nucleotide receptor mediation of membrane pore formation and superoxide generation in human promyelocytes and neutrophils. J. Immunol. 166, 6754-6763

13. Suh BC, Lee H, Jun DJ, Chun JS, Lee JH and Kim KT (2001) Inhibition of H2 histamine receptor-mediated cation channel opening by protein kinase C in human promyelocytic cells. J. Immunol. 167, 1663-1667

14. Suh BC, Kim TD, Lee JU, Seong JK and Kim KT (2001) Pharmacological characterization of adenosine receptors in PGT-b mouse pineal gland cells. Br. J. Pharmacol . 134: 132-142

15. Choi SY, Choi BH, Suh BC, Chae HD, Kim JS, Shin MJ, Kang SS, Negishi M and Kim KT (2001) Potentiation of PGE2-Mediated cAMP production during neuronal differentiation of human neuroblastoma SK-N-BE(2)C cells. J. Neurochem. 79, 303-310

16. Hur EM and Kim KT (2002) G protein coupled receptor signalling and cross-talk: achieving rapidity and specificity. Cellular Signalling 14, 397-405

17. Park TJ, Park YS, Lee TG, Ha HJ and Kim KT (2003) Inhibition of acetylcholine-mediated effects by borneol. Biochem. Pharmacol. 65(1), 83-90

18.Park TJ and Kim KT (2003) Activation of B2 Bradykinin Receptors by Neurotensin. Cellular Signalling, 15, 519-527

19. Lee IS, Hur EM, Suh BC, Kim MH, Koh DS, Rhee IJ, Ha H and Kim KT (2003) Protein kinase A- and C-induced insulin release from Ca2+-insensitive pools. Cellular Signalling, 15, 529-537

20. Lee H, Choi BH, Suh BC, Lee SK and Kim KT (2003) Attenuation of signal flow from P2Y6 receptor by PKC alpha in SK-N-BE(2)C human neuroblastoma cells. J. Neurochem. 85(4), 1043-53

21. Namgung U, Choi BH, Park SH, Lee JU, Seo HS, Suh BC and Kim KT (2004) Activation of cyclin-dependent kinase 5 is involved in axonal regeneration. Mol. Cell. Neurosci. 25(3), 422-32

22. Hur EM, Park YS, Lee BD, Jang IH, Kim HS, Kim TD, Suh PG, Ryu SH and Kim KT (2004) Sensitization of epidermal growth factor (EGF)-induced signaling by bradykinin is mediated by c-Src: implications for a role of lipid microdomains. J. Biol. Chem. 279, 5852-5860

23. Woo KC, Park YS, Jun DJ, Lim JO, Baek WY, Suh BS, and Kim KT (2004) Phytoestrogen Cimicifugoside-Mediated Inhibition of Catecholamine Secretion by Blocking Nicotinic Acetylcholine Receptor in Bovine Adrenal Chromaffin Cells. J. Pharmacol. Exp. Ther. 309(2), 641-9

24. Choi BH, Chae HD, Park TJ, Oh JS, Lim JK, Kang SS, Ha HJ, and Kim KT (2004) Protein Kinase C regulates the activity and stability of serotonin N-acetyltransferase. J. Neurochem. 90(2), 442-54

25. JH Lee and Kim KT (2004) Induction of cyclin-dependent kinase 5 and its activator p35 through the ERK and PKA pathways during retinoic-acid mediated neuronal differentiation in human neuroblastoma SK-N-BE(2)C cells. J. Neurochem. 91(3), 634-47

26. Kim TD, Kim JS, Kim JH, Myung J, Chae HD, Woo KC, Jang SK, Koh DS, and Kim KT (2005) Rhythmic Serotonin N-acetyltransferase mRNA Degradation Is Essential for the Maintenance of Its Circadian Oscillation. Mol. Cell. Biol. 25, 3232-3246